Luminol is a chemical known for its chemiluminescence. When it reacts with blood, it glows a vivid blue; as such, it is used in crime scene investigations to find traces of blood that would otherwise be hidden.
Bruce Goodman's murder[]
While investigating the murder of Bruce Goodman, Phoenix Wright was given a bottle of luminol by Ema Skye to search for traces of blood in a number of locations, including the police department's evidence room and the underground parking lot of the prosecutor's office, with the help of Skye's specialized glasses. The results proved to be very helpful, as Wright found the bloody handprint of Jake Marshall on one of the lockers in the evidence room.
Rosie Ringer's murder[]
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While investigating the murder of Rosie Ringer, Miles Edgeworth met Skye on the Bigg Building observation deck. When he deduced that the murder may have occurred in a hidden evidence room on the 51st floor, Skye gave him a bottle of luminol to search for bloodstains. Positive results on the ladder leading into the room, a chained chest, and the lift led Edgeworth to deduce that the victim's body had been hidden in the chest before then being transported via the elevator.
Murder of an Interpol agent[]
Upon discovering the body of Romein LeTouse in a Sunshine Coliseum dressing room, it was found that the victim had written something in his own blood on the floor, which the killer had then wiped until it was illegible. Ema Skye, now a police detective, gave Apollo Justice the task of testing the blood traces with luminol to see what the victim wrote. Upon applying the substance, the writing turned out to be not the killer's name, as was first thought. Instead it was a series of numbers and letters: IPXX314206. This was soon revealed to be an Interpol ID number, leading to the discovery that the victim had been an undercover agent of said organization; a vital clue that helped Justice to solve the case, clear his client's name, and catch the real murderer.
Investigation at an aquarium[]
Luminol was also used by Phoenix Wright, Pearl Fey, and Athena Cykes during the investigation into the murder of Jack Shipley at Shipshape Aquarium. The presence of a bloodstain on the skull rock decoration in the orca pool helped Wright determine that the victim had hit the rock upon falling to his death. After Fey accidentally sprayed some luminol on the aquarium's trained orca, Orla Shipley, and discovered traces of the victim's blood, Wright was led to the conclusion that the orca had been present when Shipley fell.
In real life[]
According to Ema Skye, luminol is available via mail order, and she was able to buy some as a teenager with her allowance. In the real world, it is much harder to get a hold of.